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我院林福呈团队综述线粒体自噬在植物-病原真菌生物互作中的作用
  国重室 2022-07-06  

  近日,林福呈团队以浙江省农科院(国重室)为第一通讯单位在国际自噬研究领域顶级期刊Autophagy(IF5y=16.142)上发表题为“Current opinions on mitophagy in fungi”的综述论文。该文章第一作者为沈自芳博士,通讯作者为林福呈教授,浙江省农科院李琳博士、朱学明博士,浙江大学刘小红教授和美国密西根大学Klionsky教授参与了文章的撰写与修改。该论文得到了国家自然科学基金和浙江省自然科学基金,农产品质量安全危害因子与风险防控国家重点实验室等经费的支持。

  线粒体自噬是确保线粒体质量控制的最重要的细胞过程之一,其目的是将受损、老化、功能失调或过剩的线粒体运输到液泡(植物和真菌)或溶酶体(哺乳动物)中进行降解和再利用。近年来的研究表明,线粒体自噬在病原真菌的产孢、营养生长和毒力中起着至关重要的作用,这与哺乳动物和酵母中的作用有很大的不同。文章总结展望了线粒体自噬在病原真菌中的研究现状,这将有助于研究者和读者更好地了解线粒体自噬的多重功能,并为研究线粒体自噬在真菌发病机制中的作用提供新的视角。
线粒体自噬发生的前提是待降解线粒体的准确识别。如图1所示,生物体中线粒体自噬的发生可以分别五个步骤,分别为(1)线粒体自噬受体的激活;(2)受体通过其AIM基序与核心自噬蛋白Atg8互作,招募自噬膜;(3)待降解的线粒体被自噬小体精确包裹;(4)自噬小体与液泡或溶酶体融合;(5)过量和受损的线粒体被精准降解,分解产物被释放回细胞质中再利用。

图1 线粒体分裂融合与线粒体自噬

  目前的研究表明,受体介导的线粒体自噬在真核生物中普遍保守,所有已发现的选择性自噬受体均存在能够与Atg8相互作用的AIM基序,该的特征是含有W/F/Y-X-X-L/I/V序列,其中X可以是任何酸性氨基酸(图2)。此外,线粒体自噬还与翻译后修饰过程磷酸化、线粒体分裂和融合以及线粒体呼吸链复合体III密切相关。多项证据表明磷酸化是受体介导的线粒体自噬的一个关键事件,病原真菌侵染菌丝在植物细胞中的扩展依赖于线粒体点状和丝状的形态变化。

图2 真菌中的三个线粒体自噬受体

  稻瘟病严重威胁全球水稻产量和粮食安全,自噬调控稻瘟病菌致病性的机理研究是国际研究热点。30年来,林福呈教授及其团队一直致力于稻瘟病菌致病机理和绿色防控研究,取得了国内外公认的研究成果,在Molecular Plant 、Autophagy、New Phytologist和 mBio等高水平期刊上发表了158篇有影响力的SCI论文,他引10000余次。近年来团队积极参与湘湖实验室的谋划和启动建设,在农业生物互作领域的基础理论和关键技术研究方面双向发力。2020年3月份至今,团队发表5分以上高水平文章37篇,10分以上高水平文章6篇;授权国家发明专利5项,鉴定成果1项,申请国际专利5项,为浙江坤伦有机肥料有限公司带来近1.9亿的利润。

高水平论文:
2022

1. Qing Wang, Jing Wang, Pengyun Huang, Zhicheng Huang, Yan Li, Xiaohong Liu, Fucheng Lin, Jianping Lu *。 (2022)Nucleosome assembly protein 1, Nap1, is required for the growth, development and pathogenicity of Magnaporthe oryzae. International Journal of Molecular Sciences (online)

2.  Nida Asif, Fu-Cheng Lin *, Lin Li, Xueming Zhu, Sehar Nawaz.(2022)Regulation of autophagy machinery in Magnaporthe oryzae. International Journal of Molecular Sciences (online)

3. Lin Li, Xue-MIng Zhu, Jia-Qi Wu, Na Cao, Jian-Dong Bao, Xiao-Hong Liu, Fu-Cheng Lin *.(2022) The LAMMER Kinase MoKns1 regulates growth, conidiation and pathogenicity in Magnaporthe oryzae. International Journal of Molecular Sciences (online)

4.  Zi-Fang Shen, Lin Li , Xue-Ming Zhu, Xiao-Hong Liu, Daniel J. Klionsky, Fu-Cheng Lin *. (2022)Current opinions on mitophagy in fungi. Autophagy (online)

5. Lin, Chuyu; Wu, Zhongling; Shi, Huanbin; Yu, Jinwei; Xu, Mengting; Lin, Fucheng; Kou, Yanjun; Tao, Zeng. (2022) The additional subunit of PRC2 and Sin3 histone deacetylase complex are required for the normal distribution of H3K27me3 occupancy and transcriptional silencing in Magnaporthe oryzae. New Phytologist (online)

6. Meng-Yu Liu, Li-Xiao Sun, Hui Qian, Yun-Ran Zhang, Xue-Ming Zhu, Lin Li, Shuang Liang, Jian-Ping Lu, Fu-Cheng Lin, Xiao-Hong Liu *. (2022) De novo Purine Nucleotide Biosynthesis Pathway Is Required for Development and Pathogenicity in Magnaporthe oryzae. Journal of Fungi (Basel, Switzerland) (online)

7. Lin Li, Xue-Ming Zhu, Yun-Ran Zhang, Ying-Ying Cai, Jiao-Yu Wang, Meng-Yu Liu, Jiao-Yu Wang, Jian-Dong Bao and Fu-Cheng Lin*. (2022) Research of molecular interaction mechanism between plants and pathogenic fungi. International Journal of Molecular Sciences (online)

8. Ying-Ying Cai, Jing-Yi Wang, Xi-Yu Wu, Shuang Liang, Xue-Ming Zhu, Lin Li, Jian-Ping Lu, Xiao-Hong Liu, Fu-Cheng Lin*. (2022). MoOpy2 is essential for fungal development, pathogenicity, and autophagy in Magnaporthe oryzae. Environmental Microbiology 24, 1653-1671.

9. Peng-Yun Huang, Hui-Juan Cao, Yan Li, Si-Yi Zhu, Jing Wang, Qing Wang, Xiao-Hong Liu, Fu-Cheng Lin, Jian-Ping Lu. (2022). Melanin promotes spore production in the rice blast fungus Magnaporthe oryzae. Frontiers in Microbiology 13, 843838.
10. Peng-Yun Huang, Yan Li, Jing Wang, Qing Wang, Zhi-Cheng Huang, Xiao-Hong Liu, Fu-Cheng Lin, Jian-Ping Lu. (2022). Casein kinase 2 mediates degradation of transcription factor pcf1 during appressorium formation in the rice blast fungus. Journal of Fungi (Basel, Switzerland) 8.

11. Ying-Min Qu, Hui-Juan Cao, Peng-Yun Huang, Jing Wang, Xiao-Hong Liu, Jian-Ping Lu, Fu-Cheng Lin*. (2022). A kelch domain cell end protein, PoTea1, mediates cell polarization during appressorium morphogenesis in Pyricularia oryzae. Microbiological Research 259, 126999.

12. Li-Xiao Sun, Hui Qian, Meng-Yu Liu, Ming-Hua Wu, Yun-Yun Wei, Xue-Ming Zhu, Jian-Ping Lu, Fu-Cheng Lin, Xiao-Hong Liu. (2022). Endosomal sorting complexes required for transport-0 (ESCRT-0) are essential for fungal development, pathogenicity, autophagy and ER-phagy in Magnaporthe oryzae. Environmental Microbiology 24, 1076-1092.

13. Ming-Hua Wu, Lu-Yao Huang, Li-Xiao Sun, Hui Qian, Yun-Yun Wei, Shuang Liang, Xue-Ming Zhu, Lin Li, Jian-Ping Lu, Fu-Cheng Lin, Xiao-Hong Liu. (2022). A putative D-Arabinono-1,4-lactone oxidase, MoAlo1, is required for fungal growth, conidiogenesis, and pathogenicity in Magnaporthe oryzae. Journal of Fungi (Basel, Switzerland) 8.

14. Jia-Nan Zhu, Yi-Jun Yu, Meng-Di Dai, Yu-Lan Zeng, Xuan-Jun Lu, Ling Wang, Xiao-Hong Liu, Zhen-Zhu Su, Fu-Cheng Lin*. (2022). A new species in pseudophialophora from wild rice and beneficial potential. Frontiers in Microbiology 13, 845104.

15. Li-Xiao Sun, Hui Qian, Ming-Hua Wu, Wen-Hui Zhao, Meng-Yu Liu, Yun-Yun Wei, Xue-Ming Zhu, Lin Li, Jian-Ping Lu, Fu-Cheng Lin, Xiao-Hong Liu. (2022). A subunit of ESCRT-III, MoIst1, is involved in fungal development, pathogenicity, and autophagy in Magnaporthe oryzae. Frontiers in Plant Science 13, 845139.

2021
16. Meng-Di Dai, Yu Li, Li-Xiao Sun, Fu-Cheng Lin, Xiao-Hong Liu. (2021). Isolation and functional analysis of effector proteins of Magnaporthe oryzae. Methods in Molecular Biology 2356, 199-209.
17. Meng-Di Dai, Min Wu, Yu Li, Zhen-Zhu Su, Fu-Cheng Lin, Xiao-Hong Liu. (2021). The chitin deacetylase PoCda7 is involved in the pathogenicity of Pyricularia oryzae. Microbiology Resaerch 248, 126749.
18. Daniel J Klionsky, Amal Kamal Abdel-Aziz, Sara Abdelfatah, Mahmoud Abdellatif, Asghar Abdoli, Steffen Abel, Hagai Abeliovich, Marie H Abildgaard, Yakubu Princely Abudu, …, Fu-Cheng Lin, et al. (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)(1). Autophagy 17, 1-382.
19. Lin Li, Xue-Ming Zhu, Xiao-Hong Liu, Fu-Cheng Lin*. (2021). Methods to study autophagocytosis in Magnaporthe oryzae. Methods in Molecular Biology 2356, 173-185.
20. Lin Li, Xue-Ming Zhu, Zhen-Zhu Su, Maurizio Del Poeta, Xiao-Hong Liu, and Fu-Cheng Lin*. (2021). Insights of roles played by septins in pathogenic fungi. Virulence 12, 1550-1562.
21. Ping Li, Lu-Lu Zhao, Fan Qi, Nang Myint Phyu Sin Htwe, Qiu-Ying Li, Da-Wei Zhang, Fu-Cheng Lin, Keke Shang-Guan, Yan Liang. (2021). The receptor-like cytoplasmic kinase RIPK regulates broad-spectrum ROS signaling in multiple layers of plant immune system. Molecular Plant 14, 1652-1667.
22. Fu-Cheng Lin, Huan-Bin Shi, and Xiao-Hong Liu. (2021). Similarities and differences of autophagy in mammals, plants, and microbes. Advances in Experimental Medicine and Biology 1208, 99-114.
23. Shuai Meng, Zhi-Quan Liu, Huan-Bin Shi, Zhong-Ling Wu, Jie-Hua Qiu, Hui Wen, Fu-Cheng Lin, Zeng Tao, Chao-Xi Luo, Yan-Jun Kou. (2021). UvKmt6-mediated H3K27 trimethylation is required for development, pathogenicity, and stress response in Ustilaginoidea virens. Virulence 12, 2972-2988.
24. Ying-Min Qu, Jing Wang, Peng-Yun Huang, Xiao-Hong Liu, Jian-Ping Lu, Fu-Cheng Lin*. (2021). PoRal2 is involved in appressorium formation and virulence via Pmk1 MAPK pathways in the rice blast fungus Pyricularia oryzae. Frontiers in Plant Science 12, 702368.
25. Shi-Zhen Wang, Jiao-Yu Wang, Zhen Zhang, Zhong-Na Hao, Xue-Ming Zhu, Rong-Yao Chai, Hai-Ping Qiu, Yan-Li Wang, Fu-Cheng Lin, Guo-Chang Sun. (2021). The risk of wheat blast in rice-wheat co-planting regions in China: MoO strains of Pyricularia oryzae cause typical symptom and host reaction on both wheat leaves and spikes. Phytopathology 111, 1393-1400.
26. Zhen-Zhu Su, Yu-Lan Zeng, Xiao-Li Li, Anand Babu Perumal, Jianan Zhu, Xuan-Jun Lu, Meng-Di Dai, Fu-Cheng Lin*. (2021). The endophytic fungus Piriformospora indica-assisted alleviation of cadmium in Tobacco. Journal of Fungi (Basel, Switzerland) 7.
27. Zhen-Zhu Su, Meng-Di Dai, Jia-Nan Zhu, Xiao-Hong Liu, Lin Li, Xue-Ming Zhu, Jiao-Yu Wang, Zhi-Lin Yuan, Fu-Cheng Lin*. (2021). Dark septate endophyte Falciphora oryzae-assisted alleviation of cadmium in rice. Journal of Hazardous Materials 419, 126435.
28. Zhen-Zhu Su, Meng-Di Dai, Jia-Nan Zhu, Yu-Lan Zeng, Xuan-Jun Lu, Xiao-Hong Liu, Fu-Cheng Lin*. (2021). An efficient genetic manipulation protocol for dark septate endophyte Falciphora oryzae. Biotechnology Letters 43, 2045-2052.
29. Jiao-Yu Wang, Shi-Zhen Wang, Zhen Zhang, Zhong-Na Hao, Xiao-Xiao Shi, Ling Li, Xue-Ming Zhu, Hai-Ping Qiu, Rong-Yao Chai, Yan-Li Wang, Lin Li, Xiao-Hong Liu, Xiao-Xiao Feng, Guo-Chang Sun, Fu-Cheng Lin*. (2021). MAT loci play crucial roles in sexual development but are dispensable for asexual reproduction and pathogenicity in rice blast fungus Magnaporthe oryzae. Journal of Fungi (Basel, Switzerland) 7.
30. Si-Yi Zhu, Yu-Xin Yan, Ying-Min Qu, Jing Wang, Xiao-Xiao Feng, Xiao-Hong Liu , Fu-Cheng Lin, Jian-Ping Lu. (2021). Role refinement of melanin synthesis genes by gene knockout reveals their functional diversity in Pyricularia oryzae strains. Microbiology Research 242, 126620.
31. Xue-Ming Zhu, Lin Li, Jiao-Yu Wang, Li-Li Zhao, Huan-Bin Shi, Jian-Dong Bao, Zhen-Zhu Su, Xiao-Hong Liu, Fu-Cheng Lin*. (2021). Vacuolar protein-sorting receptor MoVps13 regulates conidiation and pathogenicity in rice blast fungus Magnaporthe oryzae. Journal of Fungi (Basel, Switzerland) 7.
32. Xue-Ming Zhu, Lin Li, Ying-Ying Cai, Xi-Yu Wu, Huan-Bin Shi, Shuang Liang, Ying-Min Qu, Naweed I Naqvi, Maurizio Del Poeta, Bo Dong, Fu-Cheng Lin, Xiao-Hong Liu. (2021). A VASt-domain protein regulates autophagy, membrane tension, and sterol homeostasis in rice blast fungus. Autophagy 17, 2939-2961.

2020
33. Yu Li, Min Wu, Qin Yu, Zhen-Zhu Su, Bo Dong, Jian-Ping Lu, Fu-Cheng Lin, Qian-Sheng Liao, Xiao-Hong Liu (2020). PoMet3 and PoMet14 associated with sulfate assimilation are essential for conidiogenesis and pathogenicity in Pyricularia oryzae. Current Genetics 66, 765-774.
34. Li-Juan Liang, Rajesh Jeewon, Pem Dhandevi, Siva Sundara Kumar Durairajan, Hong-Ye Li, Fu-Cheng Lin, Hong-Kai Wang (2020). A novel species of penicillium with inhibitory effects against Pyricularia oryzae and fungal pathogens inducing citrus diseases. Frontiers in Cellular Infection Microbiology 10, 604504.
35. Ying-Min Qu, Jing Wang, Xue-Ming Zhu, Bo Dong, Xiao-Hong Liu, Jian-Ping Lu, Fu-Cheng Lin*. (2020). The P5-type ATPase Spf1 is required for development and virulence of the rice blast fungus Pyricularia oryzae. Current Genetics 66, 385-395.
36. Xun Sun, Ning Wang, Ping Li, Zhi-Yan Jiang, Xiao-Yu Liu, Meng-Cen Wang, Zhen-Zhu Su, Chu-Long Zhang, Fu-Cheng Lin, Yan Liang. (2020). Endophytic fungus Falciphora oryzae promotes lateral root growth by producing indole derivatives after sensing plant signals. Plant Cell and Environment 43, 358-373.
37. Yun-Yun Wei, Shuang Liang, Yun-Ran Zhang, Jian-Ping Lu, Fu-Cheng Lin, Xiao-Hong Liu (2020). MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae. Virulence 11, 1685-1700.

国家发明专利:
1 林福呈,苏珍珠,一种提高水稻镉耐受性和降低水稻谷粒中镉含量的方法,国家发明专利ZL2021106318681(授权公告日:2022年6月10日)
2 林福呈,苏珍珠,内生真菌FO-R20在防治水稻穗颈瘟中的应用,国家发明专利ZL2021106318662(授权公告日:2022年5月27日)
3 林福呈,苏珍珠,一张内生真菌FO-R20及其应用,国家发明专利ZL2021105299293(授权公告日:2022年5月27日)
4 林福呈,苏珍珠,朱加楠,李琳,梁岩,一种内生真菌M-B927及其应用,国家发明专利ZL2021105299096(授权公告日:2022年3月15日)
5 林福呈,苏珍珠,朱加楠,李琳,梁岩,沈坤伦,一种假瓶霉内生真菌P-B313及其应用,国家发明专利ZL202110529926.X(授权公告日:2021年11月30日)

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